Development of a new bioessai survival growth copepods : application and validation on different environmental matrices
Identifieur interne : 000223 ( France/Analysis ); précédent : 000222; suivant : 000224Development of a new bioessai survival growth copepods : application and validation on different environmental matrices
Auteurs : Teddy Lesueur [France]Source :
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Abstract
Seine estuary is one of the most polluted in the northern hemisphere largely due to its urban and agricultural activities of its watershed. Despite this the copepod E. affinis represents 90% of zooplankton biomass in the Seine estuary. The aim of the thesis was to develop a new bioassay Survival Growth copepod with the copepod E. affinis on various environmental matrices. Initially, the bioassay was developed using sediments spiked with molecules representative models of estuarine contamination (APs, PAHS and PCBs) before being validated on natural sediments of the Seine estuary. The bioassay was applied in solution 4-NP and PCB 126 and Vibrio anguillarum from the Seine estuary. A multi-generational exposure protocol was applied under the same conditions for three generations. The results showed an effect on larval development after 6 days of exposure to sediments. In solution, similar results were obtained, including, retarded growth after two days of exposure to 4-NP and PCB 126 correlated with chitobiase activity levels. However, at the end of the experiment the result is not observed. At exposition with Vibrio anguillarum, increased activity was recorded chitobiase the first days, involving an increase in growth in the sixth day. As part of the multi-generational experimenting, the copepod appears to be stabilizing at the third generation.
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Despite this the copepod E. affinis represents 90% of zooplankton biomass in the Seine estuary. The aim of the thesis was to develop a new bioassay Survival Growth copepod with the copepod E. affinis on various environmental matrices. Initially, the bioassay was developed using sediments spiked with molecules representative models of estuarine contamination (APs, PAHS and PCBs) before being validated on natural sediments of the Seine estuary. The bioassay was applied in solution 4-NP and PCB 126 and Vibrio anguillarum from the Seine estuary. A multi-generational exposure protocol was applied under the same conditions for three generations. The results showed an effect on larval development after 6 days of exposure to sediments. In solution, similar results were obtained, including, retarded growth after two days of exposure to 4-NP and PCB 126 correlated with chitobiase activity levels. However, at the end of the experiment the result is not observed. At exposition with Vibrio anguillarum, increased activity was recorded chitobiase the first days, involving an increase in growth in the sixth day. As part of the multi-generational experimenting, the copepod appears to be stabilizing at the third generation.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement><orgName><li>Université du Havre</li></orgName></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Lesueur, Teddy" sort="Lesueur, Teddy" uniqKey="Lesueur T" first="Teddy" last="Lesueur">Teddy Lesueur</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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